NUP133 Recombinant antibody

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  WB analysis using 85609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85609-1-RR (NUP133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85609-1-RR against Human NUP133 were performed. The affinity constant is below 1 pM.

NUP133 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85609-1-RR

Clone No.243092B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85609-1-RR targets NUP133 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUP133 fusion protein Ag3100
• Full Name: nucleoporin 133kDa
• Calculated Molecular Weight: 1156 aa, 129 kDa
• Observed Molecular Weight: 129 kDa
• GenBank Accession Number: BC020107
• Gene Symbol: NUP133
• Gene ID (NCBI): 55746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WUM0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.