NUP153 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP02679-1

  The mean NUP153 concentration was determined to be 30.07 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 97.51 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 33.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02679-1

  Sandwich ELISA standard curve of MP02679-1, Human NUP153 Recombinant Matched Antibody Pair - PBS only. 86622-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5519. 86622-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

NUP153 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 86622-2-PBS

Clone No.251519B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153

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Product Information

86622-2-PBS targets NUP153 as part of a matched antibody pair:

MP02679-1: 86622-1-PBS capture and 86622-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5519

  Product name: Recombinant human NUP153 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1125-1475 aa of BC052965

  Sequence: EEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK

• Full Name: nucleoporin 153kDa
• Calculated Molecular Weight: 154 kDa
• GenBank Accession Number: BC052965
• Gene Symbol: NUP153
• Gene ID (NCBI): 9972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.