NUP205 Polyclonal antibody

NUP205 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 24439-1-AP

Synonyms

205 kDa nucleoporin, C7orf14, KIAA0225, nucleoporin 205kDa, Nucleoporin Nup205, NUP205

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Validation Data Gallery

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  WB analysis of HeLa using 24439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24439-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24439-1-AP

  IP result of anti-NUP205 (IP:24439-1-AP, 4ug; Detection:24439-1-AP 1:1500) with HeLa cells lysate 4000ug.

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  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

• 

  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24439-1-AP is NUP205 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP205 fusion protein Ag19832
• Full Name: nucleoporin 205kDa
• Calculated Molecular Weight: 2012 aa, 228 kDa
• Observed Molecular Weight: 205 kDa
• GenBank Accession Number: BC136624
• Gene Symbol: NUP205
• Gene ID (NCBI): 23165
• RRID: AB_2879550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.

Publications

Species	Application	Title
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Hum Mutat; Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation.; Authors - Meng Han
mouse	IF	Cell Rep Methods; TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy; Authors - Emmanuelle Steib
human	WB,IHC	Front Oncol; Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma; Authors - Wenjia Liang