NUP53 Polyclonal antibody

NUP53 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19819-1-AP

Synonyms

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP 44, MP44, nucleoporin 35kDa, Nucleoporin Nup35, Nucleoporin NUP53, NUP35, NUP53

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Validation Data Gallery

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  WB analysis using 19819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 19819-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19819-1-AP is NUP53 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP53 fusion protein Ag13869
• Full Name: nucleoporin 35kDa
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC061698
• Gene Symbol: NUP53
• Gene ID (NCBI): 129401
• RRID: AB_2878611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.