NUP53 Polyclonal antibody

• 

  WB analysis using 19819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19819-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

NUP53 Polyclonal Antibody for WB, ELISA

Cat No. 19819-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP 44, MP44, nucleoporin 35kDa, Nucleoporin Nup35, Nucleoporin NUP53, NUP35, NUP53

---

Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19819-1-AP targets NUP53 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13869

  Product name: Recombinant human NUP35 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-326 aa of BC061698

  Sequence: MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLPAPVTPQPRSISGPSVGVMEMRSPLLAGGSPPQPVVPAHKDKSGAPPVRSIYDDISSPGLGSTPLTSRRQPNISVMQSPLVGVTSTPGTGQSMFSPASIGQPRKTTLSPAQLDPFYTQGDSLTSEDHLDDSWVTVFGFPQASASYILLQFAQYGNILKHVMSNTGNWMHIRYQSKLQARKALSKDGRIFGESIMIGVKPCIDKSVMESSDRCALSSPSLAFTPPIKTLGTPTQPGSTPRISTMRPLATAYKASTSDYQVISDRQTPKKDESLVSKAMEYMFGW

• Full Name: nucleoporin 35kDa
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC061698
• Gene Symbol: NUP53
• Gene ID (NCBI): 129401
• RRID: AB_2878611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NFH5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		bioRxiv; Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity; Authors - Katrina M Zinn