NUP62 Monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 66573-1-Ig (same clone as 66573-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66573-1-Ig (same clone as 66573-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP51499-1

  Cytometric bead array sample test of MP51499-1, NUP62 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66573-2-PBS. Detection antibody: 66573-1-PBS.

• 

  Cytometric bead array standard curve of MP51499-1

  Cytometric bead array standard curve of MP51499-1, NUP62 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66573-2-PBS. Detection antibody: 66573-1-PBS. Standard:Ag4912. Range: 0.781-100 ng/mL.

NUP62 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 66573-1-PBS

Clone No.2G12B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

2G12B3, 62 kDa nucleoporin, IBSN, Nuclear pore glycoprotein p62, nucleoporin 62kDa

---

Product Information

66573-1-PBS targets NUP62 as part of a matched antibody pair:

MP51499-1: 66573-2-PBS capture and 66573-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4912

  Product name: Recombinant human NUP62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 173-522 aa of BC014842

  Sequence: IGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTPTATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATATTTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD

• Full Name: nucleoporin 62kDa
• Calculated Molecular Weight: 522 aa, 53 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC014842
• Gene Symbol: NUP62
• Gene ID (NCBI): 23636
• RRID: AB_2881933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P37198
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)