NUP85 Polyclonal antibody

NUP85 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19370-1-AP

Synonyms

85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

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Validation Data Gallery

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  WB analysis of mouse lung using 19370-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19370-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19370-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19370-1-AP (NUP85 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19370-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19370-1-AP (NUP85 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 19370-1-AP

  IF result (Nucleus stain) of anti-NUP85 (19370-1-AP; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: mouse lung tissue, HeLa cells, human brain tissue, mouse thymus tissue
• Positive IHC detected in: human stomach tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

19370-1-AP targets NUP85 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP85 fusion protein Ag7086
• Full Name: nucleoporin 85kDa
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC000697
• Gene Symbol: NUP85
• Gene ID (NCBI): 79902
• RRID: AB_10859826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	Hum Mol Genet; Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders.; Authors - Ethiraj Ravindran
human	WB,IF	Front Microbiol; Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein; Authors - Yue-Huan Ling ; KD Validated
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB	Front Neurol; Case report: Compound heterozygous NUP85 variants cause autosomal recessive primary microcephaly; Authors - Ethiraj Ravindran