NUP88 Polyclonal antibody

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  WB analysis using 55465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55465-1-AP (NUP88 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55465-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55465-1-AP (NUP88 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

NUP88 Polyclonal Antibody for WB, ELISA

Cat No. 55465-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

88 kDa nucleoporin, nucleoporin 88kDa, Nucleoporin Nup88, NUP88

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, PC-3 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55465-1-AP targets NUP88 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nucleoporin 88kDa
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: NM_002532
• Gene Symbol: NUP88
• Gene ID (NCBI): 4927
• RRID: AB_11183758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99567
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP88 is an essential component of the nuclear pore complex. This antibody is specific to NUP88.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
mouse	WB	Biomedicines; ADAMTS4 Reduction Contributes to Extracellular Matrix Deposition and Impaired Myogenesis in the Skeletal Muscle of Cigarette Smoke-Exposed Mice; Authors - Danyang Li
mouse	WB	Cell Rep; Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis; Authors - Mei Wu