NUPL1 Polyclonal antibody

NUPL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19907-1-AP

Synonyms

KIAA0410, nucleoporin like 1, Nucleoporin like protein 1, Nucleoporin p58/p45, NUPL1, PRO2463

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Validation Data Gallery

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  WB analysis of HepG2 using 19907-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19907-1-AP (NUPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19907-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19907-1-AP (NUPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 19907-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19907-1-AP (NUPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19907-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19907-1-AP (NUPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, L02 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 19907-1-AP is NUPL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUPL1 fusion protein Ag13901
• Full Name: nucleoporin like 1
• Calculated Molecular Weight: 599 aa, 61 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC001104
• Gene Symbol: NUPL1
• Gene ID (NCBI): 9818
• RRID: AB_10694673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki