Nogo B receptor Polyclonal antibody

Nogo B receptor Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15986-1-AP

Synonyms

C6orf68, NgBR, Nogo B receptor, NUS1

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 15986-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15986-1-AP (NUS1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15986-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15986-1-AP (NUS1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15986-1-AP targets Nogo B receptor in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nogo B receptor fusion protein Ag8789
• Full Name: nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 293 aa, 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC013026
• Gene Symbol: NUS1
• Gene ID (NCBI): 116150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nogo proteins containing three isoforms (Nogo A, B, C) have been implicated in a variety of critical cellular processes including CNS neuronal regeneration, vascular cell spreading and chemotaxis. While only two functional receptors have been reported so far: NgR binding all three Nogos and NgBR specifically binding Nogo-B. Nogo-B receptor, also known as NgBR or NUS1, specifically binds the amino amino terminus (residues 1-200) of Nogo-B, the interaction of which is essential for stimulating chemotaxis, morphogenesis and angiogenesis.Western blot analysis detected high NgBR expression in mouse heart, liver, kidney, and pancreas with molecular weight around 30-33 kDa.

Publications

Species	Application	Title
mouse	WB	Front Cell Neurosci; Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR.; Authors - Christoph Eckharter