Tested Applications
| Positive WB detected in | mouse brain tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15986-1-AP targets Nogo B receptor in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8789 Product name: Recombinant human NUS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-293 aa of BC013026 Sequence: MTGLYELVWRVLHALLCLHRTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKPPAVGRNRRHHRHPRGGSCLAAAHHRMRWRADGRSLEKLPVHMGLVITEVEQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGLDCSKYSPEFANSNDKDDQVLNCHLAVKVLSPEDGKADIVRAAQDFCQLVAQKQKRPTDLDVDTLASLLSSNGCPDPDLVLKFGPVDSTLGFLPWHIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK Predict reactive species |
| Full Name | nuclear undecaprenyl pyrophosphate synthase 1 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 293 aa, 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC013026 |
| Gene Symbol | Nogo B receptor |
| Gene ID (NCBI) | 116150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96E22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nogo proteins containing three isoforms (Nogo A, B, C) have been implicated in a variety of critical cellular processes including CNS neuronal regeneration, vascular cell spreading and chemotaxis. While only two functional receptors have been reported so far: NgR binding all three Nogos and NgBR specifically binding Nogo-B. Nogo-B receptor, also known as NgBR or NUS1, specifically binds the amino amino terminus (residues 1-200) of Nogo-B, the interaction of which is essential for stimulating chemotaxis, morphogenesis and angiogenesis.Western blot analysis detected high NgBR expression in mouse heart, liver, kidney, and pancreas with molecular weight around 30-33 kDa.
Publications
What published studies show
Antibody 15986-1-AP (anti-Nogo-B) has 1 total citation. It appears in Frontiers in Cellular Neuroscience (2015), where it was used for Western Blot in mouse samples. The associated research field is neuroscience, specifically investigating how Schwann cell–expressed Nogo-B modulates axonal branching of adult sensory neurons through the Nogo-B receptor NgBR.
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR. |
