NUSAP1 Recombinant monoclonal antibody

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• NUSAP1 Recombinant monoclonal antibody (87034-3-RR)

• 

  WB analysis using 87034-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87034-3-RR (NUSAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 87034-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (87034-3-RR, Clone: 252131B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NUSAP1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87034-3-RR

Clone No.252131B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ANKT, BM037, BM-037, LNP, Nucleolar and spindle-associated protein 1

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, BGC-823 cells, ACHN cells, MDA-MB-231 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87034-3-RR targets NUSAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2654

  Product name: Recombinant human NUSAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-440 aa of BC001308

  Sequence: MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIKHEARKGNENQDESQTSASSCDETEIQISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQQNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQEAENAVSSGNRDSKVPSEGKKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFEEHNSMNELKQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAISAAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVSNKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQRKKREQERKEKKAKVLGMRRGLILAED

• Full Name: nucleolar and spindle associated protein 1
• Calculated Molecular Weight: 440 aa, 49 kDa
• Observed Molecular Weight: 49-60 kDa
• GenBank Accession Number: BC001308
• Gene Symbol: NUSAP1
• Gene ID (NCBI): 51203
• RRID: AB_3745289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BXS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.