NXF1 Polyclonal antibody

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• NXF1 Polyclonal antibody (10328-1-AP)

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• KD/KO Validated

NXF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10328-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

MEX67, mRNA export factor TAP, TAP, Tip associated protein, Tip associating protein

• 

  WB analysis of HeLa using 10328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10328-1-AP

  WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 10328-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 10328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10328-1-AP

  IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 10328-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10328-1-AP (NXF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10328-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10328-1-AP (NXF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat lung using 10328-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10328-1-AP (NXF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NXF1 antibody (10328-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, mouse stomach tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10328-1-AP targets NXF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0413

  Product name: Recombinant human NXF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 45-349 aa of BC004904

  Sequence: SGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLL

• Full Name: nuclear RNA export factor 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC004904
• Gene Symbol: NXF1
• Gene ID (NCBI): 10482
• RRID: AB_2236500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBU9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

Publications

What published studies show

The antibody Anti-NXF1 (10328-1-AP) has 16 citations published in journals including Molecular Cell, Nucleic Acids Research, Journal of Cell Biology, iScience, PLoS Genetics, Molecular and Cellular Biology, Cell Death & Discovery, Life Sciences, Chromosoma, Gene, Biochemical and Biophysical Research Communications, Acta Biochimica et Biophysica Sinica, Data in Brief, and Frontiers in Immunology. Research fields span mRNA nuclear export, nuclear pore complex biology, transcription–splicing coupling, cancer progression (endometrial, lung), spermatogenesis, aging, and RNA metabolism.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.; Authors - Suli Chen ; KD Validated
human	WB	Nucleic Acids Res; Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.; Authors - Sarah H Stubbs
		Cell Death Discov; Exon junction complex and nuclear pore complex components preferentially govern the nuclear export of spliced lncRNAs.; Authors - Sondas Saeed
human	WB	Life Sci; TRAF2 mediates Wnt-induced β-catenin nuclear translocation by associating with the nuclear pore complex; Authors - Min Yang
human	WB	J Cell Biol; Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.; Authors - Zhang Liang L ; KD Validated
		J Cell Biol; Intronless mRNAs transit through nuclear speckles to gain export competence.; Authors - Ke Wang