NXF1 Monoclonal antibody, PBS Only

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  WB analysis using 67914-1-Ig (same clone as 67914-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67914-1-Ig (NXF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67914-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67914-1-Ig (same clone as 67914-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NXF1 antibody (67914-1-Ig, Clone: 2C2H1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67914-1-PBS in a different storage buffer formulation.

NXF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67914-1-PBS

Clone No.2C2H1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

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Product Information

67914-1-PBS targets NXF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27329

  Product name: Recombinant human NXF1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 45-349 aa of BC004904

  Sequence: SGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLL

• Full Name: nuclear RNA export factor 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC004904
• Gene Symbol: NXF1
• Gene ID (NCBI): 10482
• RRID: AB_2918668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UBU9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.