NXF2 Polyclonal antibody

NXF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12185-1-AP

Synonyms

Cancer/testis antigen 39, CT39, nuclear RNA export factor 2, NXF2, NXF2B, TAP like protein 2, TAPL 2, TAPL2

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Validation Data Gallery

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  WB analysis of mouse testis using 12185-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12185-1-AP (NXF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12185-1-AP

  IP result of anti-NXF2 (IP:12185-1-AP, 4ug; Detection:12185-1-AP 1:400) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lymphoma using 12185-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12185-1-AP (NXF2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

12185-1-AP targets NXF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NXF2 fusion protein Ag2841
• Full Name: nuclear RNA export factor 2
• Calculated Molecular Weight: 626 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC015020
• Gene Symbol: NXF2
• Gene ID (NCBI): 56001
• RRID: AB_2236531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NXF2, also named as Nuclear RNA export factor 2 or TAPL2, is a 626 amino acid protein, which contains one TAP-C domain, one RRM domain, one NTF2 domain, and four LRR repeats. NXF2 belongs to the NXF family and is expressed almost exclusively in testis.NXF2 localizes in the nucleus and is involved in the export of mRNA from the nucleus to the cytoplasm.

Publications

Species	Application	Title
mouse	IF	Biochem J; Nuclear export factor 3 is involved in regulating the expression of TGF-β3 in an mRNA export activity-independent manner in mouse Sertoli cells.; Authors - Yin Yimeng Y