TXNL6 Polyclonal antibody

TXNL6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11203-1-AP

Synonyms

nucleoredoxin like 1, Nucleoredoxin like protein 1, NXNL1, RDCVF, Thioredoxin like protein 6, TXNL6

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Validation Data Gallery

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  WB analysis of mouse brain using 11203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11203-1-AP

  IP result of anti-TXNL6 (IP:11203-1-AP, 4ug; Detection:11203-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11203-1-AP (TXNL6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

11203-1-AP targets TXNL6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNL6 fusion protein Ag1691
• Full Name: nucleoredoxin-like 1
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC014127
• Gene Symbol: NXNL1
• Gene ID (NCBI): 115861
• RRID: AB_2158126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNL6, also named as NXNL1, belongs to the nucleoredoxin family. It may play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods. TXNL6 is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens. It serve as a reducing system for MsrA repair of the essential lens chaperone a-crystallin/sHSP and mitochondrial cytochrome c. TXNL6 is induced at high levels in human lens epithelial cells exposed to H2O2-induced oxidative stress.(PMID:21079812)

Publications

Species	Application	Title
pig	IF	Cell Rep; Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.; Authors - Wei Wang
human	WB,IF	PLoS One; TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of α-crystallin and cytochrome C in the eye lens.; Authors - Brennan Lisa A LA