TXNL6 Polyclonal antibody

TXNL6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11203-1-AP

Synonyms

nucleoredoxin like 1, Nucleoredoxin like protein 1, NXNL1, RDCVF, Thioredoxin like protein 6, TXNL6



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11203-1-AP targets TXNL6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNL6 fusion protein Ag1691
Full Name nucleoredoxin-like 1
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC014127
Gene Symbol NXNL1
Gene ID (NCBI) 115861
RRIDAB_2158126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNL6, also named as NXNL1, belongs to the nucleoredoxin family. It may play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods. TXNL6 is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens. It serve as a reducing system for MsrA repair of the essential lens chaperone a-crystallin/sHSP and mitochondrial cytochrome c. TXNL6 is induced at high levels in human lens epithelial cells exposed to H2O2-induced oxidative stress.(PMID:21079812)

Protocols

Product Specific Protocols
WB protocol for TXNL6 antibody 11203-1-APDownload protocol
IHC protocol for TXNL6 antibody 11203-1-APDownload protocol
IP protocol for TXNL6 antibody 11203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Cell Rep

Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.

Authors - Wei Wang
humanWB,IF

PLoS One

TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of α-crystallin and cytochrome C in the eye lens.

Authors - Brennan Lisa A LA