ChromoTek Nano-CaptureLigand™ human IgE, recombinant VHH, biotinylated

The ChromoTek Nano-CaptureLigand™ human IgE, VHH, biotinylated is used for the site-directed and specific immobilization of human IgE in biosensor and ELISA assays. It captures non-biotinylated human IgE antibodies to streptavidin/avidin. Nano-CaptureLigand human IgE, VHH, biotinylated comprises a monoclonal biotinylated VHH/ Nanobody.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Human IgE

Cat no : shuEB-1

Synonyms

Nano-CaptureLigand™ human IgE, Human IgE, Human IgE VHH, immunoglobulin epsilon

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Validation Data Gallery

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  BLI binding kinetics of a human IgE antibody. 20 nM human IgE antibody was immobilized using Nano-CaptureLigand human IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Human IgE is stably captured by the Nano-CaptureLigand with negligible dissociation.

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  BLI binding kinetics of human, mouse and rabbit antibodies. 20 nM of human, mouse and rabbit antibodies were immobilized using Nano-CaptureLigand human IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Only human IgE antibody is captured by the Nano-CaptureLigand with negligible dissociation.

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  Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) assay followed by capture of an antibody (orange). The setup is ready for characterization of the antibodies’ binding kinetics and affinity to target (red), antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

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  ELISA capture of a human IgE antibody using Nano-CaptureLigand human IgE, VHH, biotinylated. 50 nM Nano-CaptureLigand human IgE, VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgE antibody was titrated in a 1:2 dilution series and detected with a rabbit anti-human IgE primary antibody and an alkaline phosphatase-conjugated detection antibody.

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  Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

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  Species reactivity of Secondary VHH.

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Product Information

The ChromoTek Nano-CaptureLigand™ human IgE, VHH, biotinylated is used for the site-directed and specific immobilization of human IgE in biosensor and ELISA assays. It captures non-biotinylated human IgE antibodies to streptavidin/avidin. Nano-CaptureLigand human IgE, VHH, biotinylated comprises a monoclonal biotinylated VHH/ Nanobody.

• Description: The next level of antibody immobilization to BLI and SPR biosensors

  • Gentle, site-directed immobilization of human IgE antibodies to streptavidin coated biosensors without biotinylation of the antibody

  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

  • High affinity for stable baseline without significant dissociation

  • >10x regeneration for multiple reuse

  • High specificity enables human IgE antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

• Applications: BLI, SPR, ELISA
• Host: Alpaca
• Reactivity: Human IgE; Does not bind to human IgG1 (kappa and lambda LC), IgG2, IgG3, IgG4, IgA, IgM;; mouse IgE, Ig1 (kappa and lambda LC), IgG2a, IgG2b, IgG2c, IgG3; rabbit IgG
• Conjugate: Biotin
• Physical State: Liquid
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Nanobody
• Class: Recombinant
• Affinity (KD): K_D =0.72 nM; Apparent affinity may be higher for full IgEs due to avidity effects (1 antibody captured by 2 Nanobodies).
• Storage Buffer: 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
• RRID: AB_2848185
• Note: This product is for research use only, not for diagnostic or therapeutic use