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ChromoTek Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

Specificity

Fc-fragment of human IgG1, IgG2, IgG3, and IgG4

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat no : shurbGB-1

Synonyms

immunoglobulin, human IgG, Fc domain, Rabbit IgG

Validation Data Gallery

  • BLI binding kinetics of a human IgG1 anti-IgE antibody to human IgE. A human IgG1 anti-IgE antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of human IgE.

    BLI binding kinetics of a human IgG1 anti-IgE antibody to human IgE. A human IgG1 anti-IgE antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of human IgE.

  • BLI binding kinetics of different human IgG antibodies. Different monoclonal human IgG antibodies were immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. All four human IgG subclasses are stably captured by the Nano-CaptureLigand with negligible dissociation.

    BLI binding kinetics of different human IgG antibodies. Different monoclonal human IgG antibodies were immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. All four human IgG subclasses are stably captured by the Nano-CaptureLigand with negligible dissociation.

  • Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of human IgG antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

    Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of human IgG antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

  • ELISA capture of human IgG1 lambda antibody using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

    ELISA capture of human IgG1 lambda antibody using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

  • Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

    Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

  • Species reactivity of Secondary VHH.

    Species reactivity of Secondary VHH.

Product Information

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

ApplicationsBLI, SPR, ELISA
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Specificity/TargetFc-fragment of human IgG1, IgG2, IgG3, and IgG4
Fc-fragment of rabbit IgG
Conjugate1 g/L (71 μM)
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F