ChromoTek Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated
The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.
Ready to use reagent
Specificity
Fc-fragment of human IgG1, IgG2, IgG3, and IgG4
Applications
BLI, SPR, ELISA
Conjugate
Biotin
Cat no : shurbGB-1
Synonyms
Validation Data Gallery
Product Information
The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.
Applications | BLI, SPR, ELISA |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies). |
Specificity/Target | Fc-fragment of human IgG1, IgG2, IgG3, and IgG4 Fc-fragment of rabbit IgG |
Conjugate | Biotin |
Storage Buffer | 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F |
Documentation
Protocol |
---|
Protocol Nano-CaptureLigand human IgG- rabbit IgG, Fc-specific VHH, biotinylated |
Specificity: |
---|
Nano-CaptureLigands specificity overview |