ChromoTek Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

Specificity

Fc-fragment of human IgG1, IgG2, IgG3, and IgG4

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Cat no : shurbGB-1

Synonyms

immunoglobulin, human IgG, Fc domain, Rabbit IgG



Product Information

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

ApplicationsBLI, SPR, ELISA
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Specificity/TargetFc-fragment of human IgG1, IgG2, IgG3, and IgG4
Fc-fragment of rabbit IgG
ConjugateBiotin
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F