ChromoTek Nano-CaptureLigand™ mouse IgG2a, Fc-specific VHH, biotinylated
The Nano-CaptureLigand mouse IgG2a captures and immobilizes mouse IgG2a antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG2a subclass with high affinity and in a site-directed manner.
Ready to use reagent
Specificity
Fc-fragment of mouse IgG2a
Applications
BLI, SPR, ELISA
Conjugate
Biotin
Cat no : smsG2aB-1
Synonyms
Validation Data Gallery
Product Information
The Nano-CaptureLigand mouse IgG2a captures and immobilizes mouse IgG2a antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG2a subclass with high affinity and in a site-directed manner.
Applications | BLI, SPR, ELISA |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1.5 nM Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies). |
Specificity/Target | Fc-fragment of mouse IgG2a |
Conjugate | Biotin |
Storage Buffer | 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F |
Documentation
Protocol |
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Protocol Nano-CaptureLigand mouse IgG2a, Fc-specific VHH, biotinylated |
Specificity: |
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Nano-CaptureLigands specificity overview |