Napsin A Monoclonal antibody, PBS Only

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  IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60259-2-Ig (NAPSA antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60259-2-Ig (Napsin A antibody) at dilution of 1:12000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 60259-2-Ig (same clone as 60259-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (60259-2-Ig, Clone: 1H7F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 60259-2-Ig (same clone as 60259-2-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human Napsin A (60259-2-Ig, Clone:1H7F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60259-2-PBS in a different storage buffer formulation.

Napsin A Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 60259-2-PBS

Clone No.1H7F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

NAPSA, 1H7F2, Asp 4, ASP4, Aspartyl protease 4

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Product Information

60259-2-PBS targets Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9721

  Product name: Recombinant human NAPSA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-420 aa of BC017842

  Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

• Full Name: napsin A aspartic peptidase
• Calculated Molecular Weight: 420 aa, 45 kDa
• GenBank Accession Number: BC017842
• Gene Symbol: Napsin A
• Gene ID (NCBI): 9476
• RRID: AB_2881381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O96009
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).