Nectin-2/PVRL2 Polyclonal antibody
Nectin-2/PVRL2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27171-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat kidney tissue, RAW 264.7 cells, mouse kidney tissue, Jurkat cells |
Positive IHC detected in | human tonsillitis tissue, human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
27171-1-AP targets Nectin-2/PVRL2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Nectin-2/PVRL2 fusion protein Ag26040 |
Full Name | poliovirus receptor-related 2 (herpesvirus entry mediator B) |
Calculated molecular weight | 58 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | BC003091 |
Gene symbol | PVRL2 |
Gene ID (NCBI) | 5819 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.
Protocols
Product Specific Protocols | |
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WB protocol for Nectin-2/PVRL2 antibody 27171-1-AP | Download protocol |
IHC protocol for Nectin-2/PVRL2 antibody 27171-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mucosal Immunol Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury. | ||
J Transl Int Med Lipid-related protein NECTIN2 is an important marker in the progression of carotid atherosclerosis: An intersection of clinical and basic studies. | ||
iScience Proteomic and single-cell landscape reveals novel pathogenic mechanisms of HBV-infected intrahepatic cholangiocarcinoma |