Nectin-2/PVRL2 Polyclonal antibody

Nectin-2/PVRL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 27171-1-AP


CD112, Herpes virus entry mediator B, Herpesvirus entry mediator B, HVEB, Nectin-2, PRR2, PVRL2, PVRR2

Tested Applications

Positive WB detected inrat kidney tissue, RAW 264.7 cells, mouse kidney tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27171-1-AP targets Nectin-2/PVRL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin-2/PVRL2 fusion protein Ag26040
Full Name poliovirus receptor-related 2 (herpesvirus entry mediator B)
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC003091
Gene Symbol PVRL2
Gene ID (NCBI) 5819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.


Product Specific Protocols
WB protocol for Nectin-2/PVRL2 antibody 27171-1-APDownload protocol
IHC protocol for Nectin-2/PVRL2 antibody 27171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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