Tested Applications
Positive WB detected in | mouse kidney tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85845-1-RR targets Nephrin in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Nephrin fusion protein Ag18994 Predict reactive species |
Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
Calculated Molecular Weight | 1241 aa, 135 kDa |
Observed Molecular Weight | 120-130 kDa |
GenBank Accession Number | BC156935 |
Gene Symbol | Nephrin |
Gene ID (NCBI) | 4868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O60500 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).
Protocols
Product Specific Protocols | |
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WB protocol for Nephrin antibody 85845-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |