Nephrin Recombinant antibody

Nephrin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85845-1-RR
Clone No.250064G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85845-1-RR targets Nephrin in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Nephrin fusion protein Ag18994 Predict reactive species
Full Name nephrosis 1, congenital, Finnish type (nephrin)
Calculated Molecular Weight 1241 aa, 135 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC156935
Gene Symbol Nephrin
Gene ID (NCBI) 4868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60500
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Protocols

Product Specific Protocols
WB protocol for Nephrin antibody 85845-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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