Neprilysin/CD10 Recombinant monoclonal antibody

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• Neprilysin/CD10 Recombinant monoclonal antibody (84181-1-RR)

Neprilysin/CD10 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84181-1-RR Copied!

Clone No. 241322A8

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

MME, Neprilysin, 241322A8, Atriopeptidase, CALLA

• 

  WB analysis of Raji using 84181-1-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 84181-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of Human Kidney(renal cell) Cancer using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of Human Kidney(renal cell) Cancer using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) cancer slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 84181-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse kidney using 84181-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Neprilysin/CD10 antibody (84181-1-RR, Clone: 241322A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84181-1-RR against Human Neprilysin/CD10 were performed. The affinity constant is 3.07 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, Raji cells, rat kidney tissue
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue, human kidney tissue, human placenta tissue, human renal cell carcinoma tissue, human pancreas cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84181-1-RR targets Neprilysin/CD10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• RRID: AB_3671736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08473
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).