Nesprin 3 Polyclonal antibody

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• Nesprin 3 Polyclonal antibody (27132-1-AP)

Nesprin 3 Polyclonal Antibody for WB, ELISA

Cat No. 27132-1-AP Copied!

Publications (1)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

C14orf49, Nesprin 3

• 

  WB analysis of C2C12 using 27132-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 27132-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat spleen using 27132-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cells, rat spleen tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27132-1-AP targets Nesprin 3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26002

  Product name: Recombinant human C14orf49 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC146604

  Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG

• Full Name: chromosome 14 open reading frame 49
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC146604
• Gene Symbol: Nesprin 3
• Gene ID (NCBI): 161176
• RRID: AB_2880767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZMZ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Publications

What published studies show

The Nesprin 3 Polyclonal antibody (Cat# 27132-1-AP) has 1 citation published in Frontiers in Cell and Developmental Biology (2021). The cited study investigated the role of SUN5–Nesprin3 interaction in sperm head-to-tail linkage using Sun5 knockout mice, placing it within the fields of reproductive biology and nuclear envelope/cytoskeletal research. The antibody was applied via immunofluorescence (IF) in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Front Cell Dev Biol; SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.; Authors - Yunfei Zhang

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The antibody received a 5-star rating from Ning Chen (January 2023), who used it at a 1:2000 dilution for Western Blot on mouse cortex lysate (Lot 00050829). The reviewer described it as a "good antibody to probe nesprin 3," indicating reliable performance for detecting the target protein in their application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Ning (Verified Customer) (01-28-2023)

Good antibody to prob nesprin 3.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Nesprin 3 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27132-1-AP)