Nesprin 3 Monoclonal antibody, PBS Only

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  WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

Nesprin 3 Monoclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Cat No. 68420-1-PBS

Clone No.1D1G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Synonyms

1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3

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Product Information

68420-1-PBS targets Nesprin 3 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26012

  Product name: Recombinant human C14orf49 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC146604

  Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG

• Full Name: chromosome 14 open reading frame 49
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC146604
• Gene Symbol: Nesprin 3
• Gene ID (NCBI): 161176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q6ZMZ3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).