Neurofibromin 1 Polyclonal antibody

Neurofibromin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27249-1-AP

Synonyms

Neurofibromin, neurofibromin 1, NF1, NFNS, VRNF, WSS

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Validation Data Gallery

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  WB analysis using 27249-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 27249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27249-1-AP (Neurofibromin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HEK-293T cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

27249-1-AP targets Neurofibromin 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neurofibromin 1 fusion protein Ag25799
• Full Name: neurofibromin 1
• Calculated Molecular Weight: 319 kDa
• Observed Molecular Weight: 250-280 kDa
• GenBank Accession Number: M60915
• Gene Symbol: NF1
• Gene ID (NCBI): 4763
• RRID: AB_2880818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

Publications

Species	Application	Title
human	WB	Clin Transl Med; IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner; Authors - Xu Zhang
human	IHC,WB	Gynecol Oncol; Survey of NF1 inactivation by surrogate immunohistochemistry in ovarian carcinomas; Authors - Martin Köbel
human	IP, WB	Front Genet; Characterization of Two Loss-of-Function NF1 Variants in Chinese Patients and Potential Molecular Interpretations of Phenotypes.; Authors - Tingting Zhang
human	WB	Mol Genet Genomic Med; A novel mutation in NF1 gene of patient with Neurofibromatosis type 1: A case report and functional study.; Authors - Tingting Zhang
human	WB,IHC,IF	J Transl Med; The oncogenic role of NF1 in gallbladder cancer through regulation of YAP1 stability by direct interaction with YAP1; Authors - Lingxiao Zhang ; KD Validated