Neurofibromin 1 Polyclonal antibody

Neurofibromin 1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 27249-1-AP

Synonyms

Neurofibromin, neurofibromin 1, NF1, NFNS, VRNF, WSS



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27249-1-AP is Neurofibromin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurofibromin 1 fusion protein Ag25799
Full Name neurofibromin 1
Calculated molecular weight 319 kDa
Observed molecular weight 319 kDa
GenBank accession numberM60915
Gene symbol NF1
Gene ID (NCBI) 4763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Neurofibromin 1 antibody 27249-1-APDownload protocol
IHC protocol for Neurofibromin 1 antibody 27249-1-APDownload protocol
IF protocol for Neurofibromin 1 antibody 27249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Genet Genomic Med

A novel mutation in NF1 gene of patient with Neurofibromatosis type 1: A case report and functional study.

Authors - Tingting Zhang
humanIP, WB

Front Genet

Characterization of Two Loss-of-Function NF1 Variants in Chinese Patients and Potential Molecular Interpretations of Phenotypes.

Authors - Tingting Zhang