Neuromedin B Monoclonal antibody

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• Neuromedin B Monoclonal antibody (66781-1-Ig)

Neuromedin B Monoclonal Antibody for IHC, ELISA

Cat No. 66781-1-Ig Copied!

Clone No. 1G3B6

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IHC, ELISA

Synonyms

neuromedin B, NMB

• 

  IHC staining of human gliomas using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66781-1-Ig targets Neuromedin B in IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0842

  Product name: Recombinant human NMB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-154 aa of BC007407

  Sequence: MARRAGGARMFGSLLLFALLAAGVAPLSWDLPEPRSRASKIRVHSRGNLWATGHFMGKKSLEPSSPSPLGTATHTSLRDQRLQLSHDLLGILLLKKALGVSLSRPAPQIQEAAGTNTAEMTPIMGQTQQRGLDCAHPGKVLNGTLLMAPSGCKS

• Full Name: neuromedin B
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: BC007407
• Gene Symbol: NMB
• Gene ID (NCBI): 4828
• RRID: AB_2882126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08949
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in pituitary gland, hypothalamus, pancreas and stomach. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo. (PMID: 15528253, PMID: 2458345).

Publications

What published studies show

The Neuromedin B Monoclonal antibody (1G3B6), catalog number 66781-1-Ig, has 1 total citation. It appears in Annals of the New York Academy of Sciences (2011), in a study examining von Economo neurons in the frontoinsular and anterior cingulate cortex. The research field is neuroscience/neuroanatomy, specifically related to human brain structure and specialized neuronal populations.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Ann N Y Acad Sci; The von Economo neurons in the frontoinsular and anterior cingulate cortex.; Authors - John M Allman