Neuromedin B Monoclonal antibody

Neuromedin B Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1G3B6

Cat no : 66781-1-Ig

Synonyms

neuromedin B, NMB

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Validation Data Gallery

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  IHC staining of human gliomas using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66781-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66781-1-Ig targets Neuromedin B in IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neuromedin B fusion protein Ag0842
• Full Name: neuromedin B
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: BC007407
• Gene Symbol: NMB
• Gene ID (NCBI): 4828
• RRID: AB_2882126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in pituitary gland, hypothalamus, pancreas and stomach. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo. (PMID: 15528253, PMID: 2458345).

Publications

Species	Application	Title
human	IHC	Ann N Y Acad Sci; The von Economo neurons in the frontoinsular and anterior cingulate cortex.; Authors - John M Allman