NeutraControl IFN-gamma Monoclonal antibody, PBS Only

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  Non-Neutralization experiment of HT-29 cell using 69507-1-Ig (same clone as 69507-1-PBS)

  Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69507-1-Ig (same clone as 69507-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69507-1-PBS in a different storage buffer formulation.

NeutraControl IFN-gamma Monoclonal Antibody for ELISA, Non-Neutralization

Cat No. 69507-1-PBS

Clone No.2F11G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Synonyms

NeutraControl IFN gamma, IFNG, 2F11G6, IFG, IFN gamma

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69507-1-Ig

Product Information

69507-1-PBS targets IFN-gamma in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IFN-gamma protein HZ-1301
• Full Name: IFN gamma
• Gene Symbol: IFNG
• Gene ID (NCBI): 3458
• ENSEMBL Gene ID: ENSG00000111537
• Conjugate: Unconjugated
• Purification Method: Protein G purification
• Storage Buffer: PBS Only, pH 7.4.
• Storage Conditions: Store at -80°C.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody is a neutralizing control antibody for IFN gamma, the immunogen is the same as Neutrakine 69007-1-Ig but could not neutralize human IFN gamma.