NeutraKine®VEGF165/121 Monoclonal antibody

NeutraKine®VEGF165/121 Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

6C3D5

Cat no : 69025-1-Ig

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF 121, VEGF 165, VEGF A, VEGF121, VEGF165, VEGFA, VPF



Neutralization

Product Information

69025-1-Ig targets NeutraKine®VEGF165/121 in Neutralization, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human HumankineVEGF165/121 protein HZ-1038
Full Name vascular endothelial growth factor A
Gene symbol VEGFA
Gene ID (NCBI) 7422
Conjugate Unconjugated
Form Lyophilized Powder
Purification Method Protein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

This antibody is used to neutralize the bioactivity of VEGF165/121. ELISA test suggests that this antibody recognize VEGF165 and VEGF121. According to sequence similarity, it may recognize other isoforms.