Nexilin Polyclonal antibody

Nexilin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27737-1-AP

Synonyms

F actin binding protein, NELIN, Nexilin, NEXN

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Validation Data Gallery

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  WB analysis using 27737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 27737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 27737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 27737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 27737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27737-1-AP targets Nexilin in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nexilin fusion protein Ag26938
• Full Name: nexilin (F actin binding protein)
• Calculated Molecular Weight: 675 aa, 81 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC114444
• Gene Symbol: NEXN
• Gene ID (NCBI): 91624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).