Lipocalin-2/NGAL Polyclonal antibody

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  WB analysis of mouse lung using 30576-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 30576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 30576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30576-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 30576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 30576-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30576-1-AP (Lipocalin-2/Ngal antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30576-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

Lcn2, Lipocalin-2, Siderocalin LCN2, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue, mouse testis tissue
• Positive IHC detected in: mouse spleen tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Product Information

30576-1-AP targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: lipocalin 2
• Calculated Molecular Weight: 23KD
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: NM_008491.1
• Gene Symbol: NGAL
• Gene ID (NCBI): 16819
• RRID: AB_3669734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11672
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it was subsequently shown that many other types of cells, including those in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Lipocalin-2/NGAL.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	IHC	J Transl Med; Lipocalin-2 promotes NSCLC progression by activating the JAK2/STAT3 signaling pathway; Authors - Jinjin Zhang ; KO Validated