Tested Applications
| Positive WB detected in | mouse lung tissue, mouse uterus tissue |
| Positive IHC detected in | mouse spleen tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83102-1-RR targets Lipocalin-2/NGAL in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0572 Product name: Recombinant Mouse Lipocalin-2/NGAL protein (His Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-10*HIS Domain: 21-200 aa of NM_008491.1 Sequence: QDSTQNLIPAPSLLTVPLQPDFRSDQFRGRWYVVGLAGNAVQKKTEGSFTMYSTIYELQENNSYNVTSILVRDQDQGCRYWIRTFVPSSRAGQFTLGNMHRYPQVQSYNVQVATTDYNQFAMVFFRKTSENKQYFKITLYGRTKELSPELKERFTRFAKSLGLKDDNIIFSVPTDQCIDN Predict reactive species |
| Full Name | lipocalin 2 |
| Calculated Molecular Weight | 23KD |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | NM_008491.1 |
| Gene Symbol | NGAL |
| Gene ID (NCBI) | 16819 |
| RRID | AB_3670815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11672 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes mouse Lipocalin-2/NGAL.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lipocalin-2/NGAL antibody 83102-1-RR | Download protocol |
| WB protocol for Lipocalin-2/NGAL antibody 83102-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Lipocalin-2/NGAL Recombinant monoclonal antibody (230372D6), catalog number 83102-1-RR, has 2 total citations. These appear in Free Radical Biology and Medicine and Neurochemistry International. The associated research fields include cisplatin-induced acute kidney injury involving the HRI-ISR axis, and post-ischemic angiogenesis related to astrocytic HIF-1α/VEGF signaling and endothelial PI3K/Akt activation upon LCN2 inhibition.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med UBR4 attenuates cisplatin-induced acute kidney injury by regulating the HRI-ISR axis. | ||
Neurochem Int Astrocytic HIF-1α/VEGF induces endothelial PI3K/Akt activation to accelerate post-ischemic angiogenesis upon LCN2 inhibition.
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