Nir2 Polyclonal antibody

• 

  WB analysis of HepG2 using 26983-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26983-1-AP (Nir2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26983-1-AP

  IP result of anti-Nir2 (IP:26983-1-AP, 4ug; Detection:26983-1-AP 1:500) with HeLa cells lysate 2400ug.

• Featured Product
• KD/KO Validated

Nir2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 26983-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IP, CoIP, ELISA

Synonyms

DRES9, NIR 2, NIR2, PITPNM, PITPnm 1, PITPNM1, Rd9, RDGB, RDGB1, RDGBA, RDGBA1

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

26983-1-AP targets Nir2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25357

  Product name: Recombinant human PITPNM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1158-1243 aa of BC022230

  Sequence: LSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

• Full Name: phosphatidylinositol transfer protein, membrane-associated 1
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC022230
• Gene Symbol: Nir2
• Gene ID (NCBI): 9600
• RRID: AB_2880712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00562
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Virol; Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.; Authors - Hongliang Wang ; KO Validated
human	IF,WB	bioRxiv; Lipid transfer proteins initiate nuclear phosphoinositide signaling; Authors - Noah D Carrillo
human		Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta
human	CoIP	Biochim Biophys Acta Mol Cell Res; The role of Nir2, a lipid-transfer protein, in regulating endothelial cell functions; Authors - Zydrune Polianskyte-Prause