Nogo receptor/NgR1 Polyclonal antibody

Nogo receptor/NgR1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27143-1-AP

Synonyms

NGR, NgR1, Nogo 66 receptor, Nogo receptor, NOGOR, reticulon 4 receptor, RTN4R

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Validation Data Gallery

• 

  WB analysis of mouse brain using 27143-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27143-1-AP (Nogo receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

27143-1-AP targets Nogo receptor/NgR1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nogo receptor/NgR1 fusion protein Ag26021
• Full Name: reticulon 4 receptor
• Calculated Molecular Weight: 473 aa, 51 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC011787
• Gene Symbol: RTN4R
• Gene ID (NCBI): 65078
• RRID: AB_2918118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nogo receptor (NgR), also known as reticulon 4 receptor (RTN4R) or Nogo-66 Receptor, is a glycosylphosphatidylinositol (GPI)-linked, leucine-rich repeat (LRR) protein (PMID: 11201742). NgR is expressed predominantly by neurons in the brain. It is the receptor of Nogo-A (Nogo-66), oligodendrocyte myelin protein (OMgp) and myelin-associated glycoprotein (MAG), and mediates inhibition of axonal regeneration (PMID: 12694398). The apparent molecular wight of NgR detected by this antibody is 64 kDa, which is larger than the calculated molecular weight of NgR, probably due to glycosylation (PMID: 15751227; 12694398).

Publications

Species	Application	Title
human	WB	J Ethnopharmacol; Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis.; Authors - Rongchun Wang
mouse,rat	WB	Cell Transplant; Human Umbilical Cord Mesenchymal Stem Cells Improve the Status of Hypoxic/Ischemic Cerebral Palsy Rats by Downregulating NogoA/NgR/Rho Pathway; Authors - Yaoling Luo