NRF2/NFE2L2 (mouse) Polyclonal antibody

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• NRF2/NFE2L2 (mouse) Polyclonal antibody (33123-1-AP)

NRF2/NFE2L2 (mouse) Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33123-1-AP

Publications(85)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nfe2l2, NF-E2-related factor 2, NFE2-related factor 2, Nrf2, Nuclear factor erythroid 2-related factor 2

• 

  WB analysis using 33123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33123-1-AP (Nrf2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 33123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33123-1-AP (Nrf2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 33123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33123-1-AP (Nrf2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 33123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33123-1-AP (Nrf2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 33123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Nrf2 antibody (33123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, NR8383 cells, rat liver tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33123-1-AP targets NRF2/NFE2L2 (mouse) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag39859

  Product name: Recombinant mouse Nfe2l2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 400-597 aa of NM_010902.4

  Sequence: SPAQGHSAPMRESQCENTTKKEVPVSPGHQKAPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVDDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLGHLKDEREKLLREKGENDRNLHLLKRRLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDTKKN

• Full Name: nuclear factor, erythroid derived 2, like 2
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 95-110 kDa, 75 kDa
• GenBank Accession Number: NM_010902.4
• Gene Symbol: Nfe2l2
• Gene ID (NCBI): 18024
• RRID: AB_3742863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q60795
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; Uncovering spatially resolved functional genomics with CRISPR screen sequencing.; Authors - Haorui Zhang
mouse	WB	Chem Biodivers; Exploring the Active Substances and Mechanism of Physalis Calyx seu Fructus Against Inflammation-related Respiratory Diseases via Integrating Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry, Network Pharmacological Analysis, and Experimental Verification.; Authors - Hui Meng
		Food Sci Biotechnol; Ethanol extract of Sauropus spatulifolius and its active constituents attenuate LPS-induced acute lung injury via NF-κB inhibition and Nrf2 activation.; Authors - Lirong Zhao
rat	IHC	Mol Biol Rep; Lutein attenuates sodium fluoride (NaF) and gamma-irradiation induced hepatotoxicity via AMPK-PGC1α mitochondrial biogenesis induction.; Authors - Amira Abd-Elraouf
mouse	WB	Exp Cell Res; Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells.; Authors - Fan Yang
rat	WB,IHC,IF	Naunyn Schmiedebergs Arch Pharmacol; Atractylenolide III improves ovarian dysfunction and endometrial receptivity in a PCOS rat model by regulating Cybb-mediated oxidative stress.; Authors - Chunmei Bai