Cat no : acg-n
DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.
|Applications||Live cell imaging|
|Specificity||Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii|
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Encoded Nanobody/ VHH||Monoclonal anti-Actin single domain antibody (sdAb) fragment|
|Sequence||With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Storage||Shipped at ambient temperature. Store at -20 °C|
|Product brochure (PDF)|
|Datasheet Actin-Chromobody? TagGFP plasmid (PDF)|
|Datasheet Actin-Chromobody? TagRFP plasmid (PDF)|
|Datasheet Nuclear Actin-Chromobody? TagGFP plasmid (PDF)|
|SDS Actin-Chromobody? TagGFP plasmid (PDF)|
|SDS Nuclear Actin-Chromobody? TagGFP plasmid (PDF)|
Cell Death Dis
Targeting actin inhibits repair of doxorubicin-induced DNA damage: a novel therapeutic approach for combination therapy.
Nat Struct Mol Biol
The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling.
Nat Cell Biol
Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.
Cytoplasmic control of intranuclear polarity by human cytomegalovirus.