ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Actin

Cat no : acg-n

Synonyms



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

Applications Live cell imaging
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Actin single domain antibody (sdAb) fragment
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle
IF

Cell Death Dis

Targeting actin inhibits repair of doxorubicin-induced DNA damage: a novel therapeutic approach for combination therapy.

Authors - Lisa Pfitzer

Nat Struct Mol Biol

The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling.

Authors - Kilian R Knoll

Nat Cell Biol

Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.

Authors - Noa Lamm

Nature

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Authors - Dean J Procter