OAS3 Antibody

OAS3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21915-1-AP

Synonyms

(2 5)oligo(A) synthase 3, 2 5 oligoadenylate synthase 3, 2 5A synthase 3, OAS3, p100, p100 OAS, p100OAS



Tested Applications

Positive WB detected inA375 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman skin cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 21915-1-AP immunogen is Fusion Protein OAS3 protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OAS3 fusion protein Ag16150
Full Name 2'-5'-oligoadenylate synthetase 3, 100kDa
Calculated molecular weight 1087aa,121 kDa
Observed molecular weight 100-120 kDa
GenBank accession number BC113746
Gene symbol OAS3
Gene ID (NCBI) 4940
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS3, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). It is also named as p100 OAS and belongs to the 2-5A synthase family. OAS3 is involved in inhibition of cellular protein synthesis and in viral infection resistance. This antibody may have weak cross-reaction with OAS1 protein due to the highly homology.

Protocols

Product Specific Protocols
WB protocol for OAS3 antibody 21915-1-APDownload protocol
IHC protocol for OAS3 antibody 21915-1-APDownload protocol
IF protocol for OAS3 antibody 21915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols