OAT Polyclonal antibody

OAT Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17089-1-AP

Synonyms

Ornithine aminotransferase, hepatic form, OKT, OATASE, HOGA, GACR

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Validation Data Gallery

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  IHC staining of human liver cancer using 17089-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17089-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17089-1-AP (OAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17089-1-AP (OAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OAT antibody (17089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17089-1-AP targets OAT in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAT fusion protein Ag9981
• Full Name: ornithine aminotransferase (gyrate atrophy)
• Calculated Molecular Weight: 62 kDa
• GenBank Accession Number: BC000964
• Gene Symbol: OAT
• Gene ID (NCBI): 4942
• RRID: AB_2878347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.