OCIAD1 Polyclonal antibody

OCIAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16634-1-AP

Synonyms

Asrij, OCIA, OCIA domain containing 1, OCIAD1, TPA018

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Validation Data Gallery

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  WB analysis using 16634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16634-1-AP (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16634-1-AP

  IP result of anti-OCIAD1 (IP:16634-1-AP, 4ug; Detection:16634-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human liver cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16634-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16634-1-AP

  Immunofluorescent analysis of HepG2 cells, using OCIAD1 antibody 16634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, human placenta tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue, human pancreas cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16634-1-AP targets OCIAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OCIAD1 fusion protein Ag9977
• Full Name: OCIA domain containing 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 29-35 kDa
• GenBank Accession Number: BC003409
• Gene Symbol: OCIAD1
• Gene ID (NCBI): 54940
• RRID: AB_2156297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

Publications

Species	Application	Title
human	WB,IF	PLoS One; OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease.; Authors - Huong T L Tran
human	IHC	Pathol Int; Increased expression of OCIA domain containing 2 during stepwise progression of ovarian mucinous tumor.; Authors - Nagata Chigusa C