OCT1 Recombinant antibody

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  WB analysis using 85726-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85726-1-RR (OCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85726-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCT1 antibody (85726-1-RR, Clone: 243074D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85726-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85726-1-RR against Human OCT1 were performed. The affinity constant is below 1 pM.

OCT1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85726-1-RR

Clone No.243074D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NF A1, NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, NCCIT cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85726-1-RR targets OCT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OCT1 fusion protein Ag0586
• Full Name: POU class 2 homeobox 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003571
• Gene Symbol: POU2F1
• Gene ID (NCBI): 5451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14859
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].