ODC1 Polyclonal antibody

ODC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (2)

Cat no : 28728-1-AP

Synonyms

ODC, EC:4.1.1.17

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Validation Data Gallery

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  WB analysis using 28728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28728-1-AP (ODC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 28728-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 28728-1-AP (ODC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 28728-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 28728-1-AP (ODC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28728-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 28728-1-AP (ODC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of LNCaP using 28728-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using ODC1 antibody (28728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse thymus tissue, HepG2 cells, LNCaP cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

28728-1-AP targets ODC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODC1 fusion protein Ag29751
• Full Name: ornithine decarboxylase 1
• Calculated Molecular Weight: 461 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC025296
• Gene Symbol: ODC1
• Gene ID (NCBI): 4953
• RRID: AB_2881200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

Publications

Species	Application	Title
human	WB	Mol Ther; Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer.; Authors - Daryl Jin Tai Tay
human	WB	Front Oncol; CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.; Authors - Lin Qiu
human	IHC	Hum Mol Genet; Ornithine Decarboxylase, the rate-limiting enzyme of polyamine synthesis, modifies brain pathology in a mouse model of tuberous sclerosis complex.; Authors - David Kapfhamer
mouse	WB	Biochim Biophys Acta Mol Cell Res; Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation.; Authors - Jiajing Li
human	WB	Onco Targets Ther; Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells.; Authors - Zhirui Zeng
pig	WB	Vet Microbiol; Polyamine regulation of porcine reproductive and respiratory syndrome virus infection depends on spermidine-spermine acetyltransferase 1.; Authors - Yanrong Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (09-07-2023)

Works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 or 1:2000
• Cell Tissue Type: Hela, HepG2, mouse liver, mouse brain

ODC1 Antibody Western Blot validation (1:1000 or 1:2000 dilution) in Hela, HepG2, mouse liver, mouse brain (Cat no:28728-1-AP)

FH

Janelle (Verified Customer) (09-12-2022)

Highly specific to target, clean blots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000