OGDH Polyclonal antibody

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• OGDH Polyclonal antibody (15212-1-AP)

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• KD/KO Validated

OGDH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15212-1-AP Copied!

Publications (108)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, Alpha-ketoglutarate dehydrogenase, Alpha-KGDH-E1, E1o

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  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 15212-1-AP

  WB result of OGDH antibody (15212-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OGDH transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat lung using 15212-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse heart using 15212-1-AP

  IP result of anti-OGDH (IP:15212-1-AP, 4ug; Detection:15212-1-AP 1:20000) with mouse heart tissue lysate 1920 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells stained for OGDH rabbit pAb (15212-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15212-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG (H+L) for 66031-1-Ig. .

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 15212-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using OGDH antibody (15212-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse heart tissue, Calu-1 cells, mouse lung tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15212-1-AP targets OGDH in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, chicken, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7368

  Product name: Recombinant human OGDH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 674-1023 aa of BC004964

  Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS

• Full Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC004964
• Gene Symbol: OGDH
• Gene ID (NCBI): 4967
• RRID: AB_2156759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02218
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Publications

What published studies show

The OGDH Polyclonal antibody (Cat# 15212-1-AP) has accumulated 108 citations. Publications span journals including Nature, Cell Metabolism, Cell Research, Nature Cell Biology, Cancer Research, Molecular Cell, Nature Communications, EMBO Journal, and PNAS. Research fields cover cancer metabolism, mitochondrial biology, neurodegeneration, immunology, cardiovascular disease, and metabolic reprogramming. Primary applications include Western blot, IHC, IF, IP, and Co-IP, tested across human, mouse, rat, and other species.

Automatically generated from the 108 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Nature; α-Ketoglutarate links p53 to cell fate during tumour suppression.; Authors - John P Morris ; KD Validated
mouse	WB	Nature; Metabolic adaptations direct cell fate during tissue regeneration; Authors - Almudena Chaves-Perez
mouse	WB	Mol Cancer; CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.; Authors - Shengfei He ; KO Validated
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse,human	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang