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OGDH Antibody

OGDH Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15212-1-AP

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue, mouse lung tissue, mouse pancreas tissue, A549 cells, rat heart tissue, rat lung tissue, ROS1728 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15212-1-AP targets OGDH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGDH fusion protein Ag7368
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession number BC004964
Gene symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Protocols

Product Specific Protocols
WB protocol for OGDH antibody 15212-1-APDownload protocol
IHC protocol for OGDH antibody 15212-1-APDownload protocol
IF protocol for OGDH antibody 15212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
  • KD Validated
humanWB

Cell Rep

Regional Molecular Mapping of Primate Synapses during Normal Healthy Aging.

Authors - Laura C Graham
mouseWB

Neurotherapeutics

Allopregnanolone Reverses Bioenergetic Deficits in Female Triple Transgenic Alzheimer's Mouse Model.

Authors - Tian Wang
mouseWB,IHC

Biochem Biophys Res Commun

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Authors - Weiwei Jin
mouseWB

Brain Res

Potentiation of brain mitochondrial function by S-equol and R/S-equol estrogen receptor β-selective phytoSERM treatments.

Authors - Yao Jia J
humanWB

J Ethnopharmacol

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Authors - Li Wu