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OGDH Polyclonal antibody

OGDH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15212-1-AP

Synonyms

EC:1.2.4.2, E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial



Tested Applications

Positive WB detected inmouse heart tissue, A549 cells, mouse liver tissue, mouse pancreas tissue, ROS1728 cells, rat heart tissue, mouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15212-1-AP targets OGDH in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, chicken, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OGDH fusion protein Ag7368
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_2156759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Protocols

Product Specific Protocols
WB protocol for OGDH antibody 15212-1-APDownload protocol
IHC protocol for OGDH antibody 15212-1-APDownload protocol
IF protocol for OGDH antibody 15212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Nature

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Authors - John P Morris
  • KD Validated
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
human,mouseWB

Cell Metab

An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.

Authors - Nunu Huang
humanWB

Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanWB

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai