OGFR Polyclonal antibody

• Featured Product
• KD/KO Validated

OGFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11177-1-AP

Synonyms

Zeta-type opioid receptor, Zeta type opioid receptor, Protein 7-60, Protein 7 60, Opioid growth factor receptor

---

Validation Data Gallery

• 

  WB analysis of COLO 320 using 11177-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11177-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11177-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11177-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 11177-1-AP

  IP result of anti-OGFR (IP:11177-1-AP, 5ug; Detection:11177-1-AP 1:500) with COLO 320 cells lysate 1200ug.

• 

  IHC staining of human colon cancer using 11177-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11177-1-AP (OGFR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COLO 320 using 11177-1-AP

  Immunofluorescent analysis of COLO 320 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of L02 using 11177-1-AP

  Immunofluorescent analysis of L02 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, A549 cells, HEK-293 cells, L02 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: COLO 320 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 10 publications below
• IF: See 1 publications below

Product Information

11177-1-AP targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OGFR fusion protein Ag1671
• Full Name: opioid growth factor receptor
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: BC014137
• Gene Symbol: OGFR
• Gene ID (NCBI): 11054
• RRID: AB_2156772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy.; Authors - Cihang Liu ; KD Validated
human	IHC	Front Oncol; Opioid Receptor Expression in Colorectal Cancer: A Nested Matched Case-Control Study.; Authors - Amparo Belltall
human	IHC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
human	WB, IHC	Int Immunopharmacol; Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB, IHC, IF	Int Immunopharmacol; Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma.; Authors - Xueli Bai
human	WB,IHC	Int Immunopharmacol; A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment.; Authors - Shuling Zhang ; KD Validated