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OGFR Polyclonal antibody

OGFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11177-1-AP

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis of COLO 320 using 11177-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of L02 using 11177-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 11177-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 11177-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11177-1-AP (OGFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of COLO 320 using 11177-1-AP
IP result of anti-OGFR (IP:11177-1-AP, 5ug; Detection:11177-1-AP 1:500) with COLO 320 cells lysate 1200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 11177-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11177-1-AP (OGFR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 11177-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGFR antibody (11177-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of L02 using 11177-1-AP
Immunofluorescent analysis of L02 cells using 11177-1-AP (OGFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells, A549 cells, HEK-293 cells, L02 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human colon cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11177-1-AP targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1671 Product name: Recombinant human OGFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-658 aa of BC014137 Sequence: LERSQGDEAGGHGEDRPEPLSPKESKKRKLELSRREQPPTEPGPQSASEVEKIALNLEGCALSQGSLRTGTQEVGGQDPGEAVQPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGHPKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP Predict reactive species
Full Name	opioid growth factor receptor
Calculated Molecular Weight	73 kDa
Observed Molecular Weight	90-110 kDa
GenBank Accession Number	BC014137
Gene Symbol	OGFR
Gene ID (NCBI)	11054
RRID	AB_2156772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZT2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for OGFR antibody 11177-1-AP	Download protocol
IHC protocol for OGFR antibody 11177-1-AP	Download protocol
IP protocol for OGFR antibody 11177-1-AP	Download protocol
WB protocol for OGFR antibody 11177-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-OGFR antibody (Cat# 11177-1-AP) has 16 citations across journals including International Immunopharmacology, Nature Communications, International Journal of Biological Sciences, International Journal of Molecular Sciences, Frontiers in Oncology, PLoS ONE, Cancer Management and Research, and American Journal of Clinical Experimental Urology. Research fields span cancer biology (lung, gastric, colorectal, cervical, prostate, osteosarcoma), opioid receptor signaling, tumor microenvironment immunology, apoptosis, autophagy, and noncoding RNA/splicing regulation.

Species	Application	Title
human	WB	Int J Biol Sci CIRBP-OGFR axis safeguards against cardiomyocyte apoptosis and cardiotoxicity induced by chemotherapy. Authors - Cihang Liu KD Validated View Article
human	WB,IHC	Int Immunopharmacol Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma. Authors - Xueli Bai View Article
human	WB,IHC	Int Immunopharmacol A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment. Authors - Shuling Zhang KD Validated View Article
human	WB,IHC,IF	Int Immunopharmacol Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma. Authors - Xueli Bai View Article
human	IHC	Int Immunopharmacol Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor. Authors - Na Qu View Article
human	IHC	Int Immunopharmacol Methionine enkephalin (MENK) inhibits human gastric cancer through regulating tumor associated macrophages (TAMs) and PI3K/AKT/mTOR signaling pathway inside cancer cells. Authors - Xiaonan Wang View Article