OGG1 Recombinant monoclonal antibody, PBS Only

• 

  IF Staining of HepG2 using 83973-4-RR (same clone as 83973-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGG1 antibody (83973-4-RR, Clone: 240940D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83973-4-RR (same clone as 83973-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ogg1 Recombinant Antibody (83973-4-RR, Clone:240940D4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83973-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83973-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83973-4-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

OGG1 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83973-4-PBS

Clone No.240940D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase

---

Product Information

83973-4-PBS targets OGG1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7204

  Product name: Recombinant human OGG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-345 aa of BC000657

  Sequence: MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

• Full Name: 8-oxoguanine DNA glycosylase
• Calculated Molecular Weight: 22 kDa, 36-40 kDa, 45-57 kDa
• GenBank Accession Number: BC000657
• Gene Symbol: OGG1
• Gene ID (NCBI): 4968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15527
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).