OGG1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83973-5-RR (same clone as 83973-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83973-5-RR (OGG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83973-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83973-5-RR (same clone as 83973-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83973-5-RR (OGG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83973-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83973-5-RR (same clone as 83973-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (83973-5-RR, Clone: 240940H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83973-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83973-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83973-5-RR against Human OGG1 were performed. The affinity constant is below 1 pM.

OGG1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 83973-5-PBS

Clone No.240940H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

240940H3, 8 oxoguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

---

Product Information

83973-5-PBS targets OGG1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7204

  Product name: Recombinant human OGG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-345 aa of BC000657

  Sequence: MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

• Full Name: 8-oxoguanine DNA glycosylase
• Calculated Molecular Weight: 22 kDa, 36-40 kDa, 45-57 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC000657
• Gene Symbol: OGG1
• Gene ID (NCBI): 4968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15527
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base by product that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa).