Osteoglycin Polyclonal antibody

Osteoglycin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 12755-1-AP

Synonyms

OGN, SLRR3A, Osteoinductive factor, OIF, OG

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 12755-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12755-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12755-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoglycin antibody (12755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, human liver tissue
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12755-1-AP targets Osteoglycin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Osteoglycin fusion protein Ag3484
• Full Name: osteoglycin
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC037273
• Gene Symbol: OGN
• Gene ID (NCBI): 4969
• RRID: AB_10646479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Publications

Species	Application	Title
human	IHC, IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
mouse	IHC	Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Onco Targets Ther; Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway.; Authors - Tao Xu
human	WB	Front Immunol; Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics; Authors - Wei Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Huai-Chin (Verified Customer) (10-24-2022)

Multiple bands in Western blot. There are two dominant bands at ~40KD and ~55KD. The one ~40KD maybe the band of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: used on both mouse and human cell lines