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OGT Polyclonal antibody
OGT Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, CoIP, IP-MS, ELISA
Conjugate
Unconjugated
Cat no : 11576-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, mouse liver tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 11 publications below |
WB | See 54 publications below |
IHC | See 13 publications below |
IF | See 8 publications below |
IP | See 7 publications below |
CoIP | See 4 publications below |
Product Information
11576-2-AP targets OGT in WB, IHC, IF, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | OGT fusion protein Ag2160 |
Full Name | O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) |
Calculated Molecular Weight | 1046 aa, 117 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC014434 |
Gene Symbol | OGT |
Gene ID (NCBI) | 8473 |
RRID | AB_2156943 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.
Protocols
Product Specific Protocols | |
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WB protocol for OGT antibody 11576-2-AP | Download protocol |
IHC protocol for OGT antibody 11576-2-AP | Download protocol |
IP protocol for OGT antibody 11576-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
J Clin Invest Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation | ||
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Nat Chem Biol Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase. | ||
Nat Chem Biol O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response. | ||
Nat Chem Biol Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (06-02-2023) | Works well for WB, dilution 1:1000
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FH Isha (Verified Customer) (05-18-2022) | Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IHC
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