OGT Polyclonal antibody

• Featured Product
• KD/KO Validated

OGT Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, IP-MS, ELISA

Conjugate

Unconjugated

Publications

64

Reviews (2)

Cat no : 11576-2-AP

Synonyms

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1

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Validation Data Gallery

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  WB analysis using 11576-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11576-2-AP (OGT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11576-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP (OGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11576-2-AP

  IP result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human colon cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11576-2-AP (OGT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11576-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11576-2-AP (OGT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 54 publications below
• IHC: See 13 publications below
• IF: See 8 publications below
• IP: See 7 publications below
• CoIP: See 4 publications below

Product Information

11576-2-AP targets OGT in WB, IHC, IF, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OGT fusion protein Ag2160
• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• RRID: AB_2156943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Nat Chem Biol; Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.; Authors - Chia-Wei Hu
mouse	WB	Nat Chem Biol; O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response.; Authors - Xingqian Zhang
human,mouse	WB,IP	Nat Chem Biol; Dynamic eIF3a O-GlcNAcylation controls translation reinitiation during nutrient stress.; Authors - Xin Erica Shu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well for WB, dilution 1:1000

• Applications: Western Blot
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

FH

Isha (Verified Customer) (05-18-2022)

Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IHC

• Applications: Western Blot, Immunohistochemistry

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