OGT Recombinant monoclonal antibody, PBS Only (Detector)

• 

  Sandwich ELISA standard curve of MP00167-1

  Sandwich ELISA standard curve of MP00167-1, Human OGT Recombinant Matched Antibody Pair - PBS only. 83113-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2160. 83113-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

• 

  Sample test of MP00167-1

  The mean OGT concentration was determined to be 2.29 ng/mL in PC-3 cell extract based on a 2.00 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP00167-1

  Cytometric bead array standard curve of MP00167-1, OGT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83113-3-PBS. Detection antibody: 83113-2-PBS. Standard: Ag2160. Range: 0.625-20 ng/mL.

OGT Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83113-2-PBS

Clone No.230385C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O GLCNAC, HRNT1, EC:2.4.1.255

---

Product Information

83113-2-PBS targets OGT as part of a matched antibody pair:

MP00167-1: 83113-3-PBS capture and 83113-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2160

  Product name: Recombinant human OGT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC014434

  Sequence: MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQD

• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15294
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.