hIST1 Polyclonal antibody

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• hIST1 Polyclonal antibody (19842-1-AP)

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19842-1-AP Copied!

Publications (17)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Synonyms

IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog

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  WB analysis using 19842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 19842-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 19842-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 19842-1-AP

  IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 19842-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using hIST1 antibody (19842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
• Positive IHC detected in: mouse stomach tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13874

  Product name: Recombinant human hIST1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-335 aa of BC000116

  Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT

• Full Name: KIAA0174
• Calculated Molecular Weight: 364 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000116
• Gene Symbol: hIST1
• Gene ID (NCBI): 9798
• RRID: AB_2878612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53990
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The hIST1 (IST1) Polyclonal antibody (Cat# 19842-1-AP) has 17 citations. These appear in journals including Nature, Science, Cell Research, Autophagy, Nature Communications, PNAS, Developmental Cell, Cell Reports, EMBO Reports, mBio, Journal of Neurochemistry, Traffic, iScience, and bioRxiv. Research fields span lysosomal/endosomal membrane repair, ESCRT machinery, autophagy, cancer progression, and neurobiology.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	IF	Cell Res; Repair of damaged lysosomes by TECPR1-mediated membrane tubulation during energy crisis.; Authors - Hanmo Chen
human	WB,IF,CoIP	Autophagy; ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms; Authors - Zhijie Xu
human	IF	Nat Commun; Impaired α -Synuclein aggregate clearance in neuronal cells drive their spread to microglia through tunneling nanotubes.; Authors - Ranabir Chakraborty
		Nat Commun; VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair.; Authors - Oluwatobi Andrew Adeosun

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single available review rates the product 5 out of 5 stars. The reviewer found it performed very well for immunofluorescence, using a 1:1000 dilution on HAP1 and eHAP cells. Overall, the feedback is positive, highlighting strong IF performance at a standard dilution.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Lianna (Verified Customer) (07-17-2025)

Looks very good for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1, eHAP