hIST1 Polyclonal antibody

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  WB analysis using 19842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19842-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19842-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19842-1-AP

  IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse lung using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19842-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using hIST1 antibody (19842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19842-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Synonyms

IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
• Positive IHC detected in: mouse stomach tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13874

  Product name: Recombinant human hIST1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-335 aa of BC000116

  Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT

• Full Name: KIAA0174
• Calculated Molecular Weight: 364 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000116
• Gene Symbol: hIST1
• Gene ID (NCBI): 9798
• RRID: AB_2878612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53990
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	IF	Proc Natl Acad Sci U S A; Ca2+-sensor ALG-2 engages ESCRTs to enhance lysosomal membrane resilience to osmotic stress; Authors - Wei Chen
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human		MBio; Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.; Authors - Ekansh Mittal
mouse	WB,RIP,IF	J Neurochem; Reduced circ_lrrc49 in trigeminal ganglion contributes to neuropathic pain in mice by downregulating Ist1 and impairing autophagy; Authors - Qingfeng Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lianna (Verified Customer) (07-17-2025)

Looks very good for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1, eHAP