hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 19842-1-AP

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hIST1 fusion protein Ag13874
Full Name KIAA0174
Calculated Molecular Weight 364 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000116
Gene Symbol hIST1
Gene ID (NCBI) 9798
RRIDAB_2878612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for hIST1 antibody 19842-1-APDownload protocol
IHC protocol for hIST1 antibody 19842-1-APDownload protocol
IF protocol for hIST1 antibody 19842-1-APDownload protocol
IP protocol for hIST1 antibody 19842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
humanIF

Proc Natl Acad Sci U S A

Ca2+-sensor ALG-2 engages ESCRTs to enhance lysosomal membrane resilience to osmotic stress

Authors - Wei Chen
humanWB

Dev Cell

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Authors - Oliver B Davis
human

MBio

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

Authors - Ekansh Mittal
mouseWB,IF,RIP

J Neurochem

Reduced circ_lrrc49 in trigeminal ganglion contributes to neuropathic pain in mice by downregulating Ist1 and impairing autophagy

Authors - Qingfeng Tang
humanWB,IF,CoIP

Autophagy

ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms

Authors - Zhijie Xu