OLIG2 Monoclonal antibody
OLIG2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E8C5
Cat no : 66513-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, human brain tissue, human cerebellum tissue, rat cerebellum tissue, mouse brain tissue |
Positive IHC detected in | human brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:350-1:1400 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 8 publications below |
Product Information
66513-1-Ig targets OLIG2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | OLIG2 fusion protein Ag18838 |
Full Name | oligodendrocyte lineage transcription factor 2 |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 35-39 kDa |
GenBank accession number | BC047511 |
Gene symbol | OLIG2 |
Gene ID (NCBI) | 10215 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )
Protocols
Product Specific Protocols | |
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WB protocol for OLIG2 antibody 66513-1-Ig | Download protocol |
IHC protocol for OLIG2 antibody 66513-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Glia Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation | ||
Front Cell Dev Biol Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area. | ||
Front Aging Neurosci Effect of β-hydroxybutyrate on behavioral alterations, molecular and morphological changes in CNS of multiple sclerosis mouse model | ||
Exp Neurol Role of TREM-1 in the development of early brain injury after subarachnoid hemorrhage. | ||
iScience Hypoxia-induced HMGB1 promotes glioma stem cells self-renewal and tumorigenicity via RAGE |