OLIG2 (for WB only) Monoclonal antibody

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• OLIG2 (for WB only) Monoclonal antibody (66513-1-Ig)

OLIG2 (for WB only) Monoclonal Antibody for WB, ELISA

Cat No. 66513-1-Ig Copied!

Clone No. 1E8C5

Publications (24)

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Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

OLIG2, 1E8C5, 5O7, bHLHb1, bHLHe19

• 

  WB analysis of human brain using 66513-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human cerebellum using 66513-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, human cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66513-1-Ig targets OLIG2 (for WB only) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18838

  Product name: Recombinant human OLIG2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-323 aa of BC047511

  Sequence: MDSDASLVSSRPSSPEPDDLFLPARSKGSSGSAFTGGTVSSSTPSDCPPELSAELRGAMGSAGAHPGDKLGGSGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKITTLLLARNYILMLTNSLEEMKRLVSEIYGGHHAGFHPSACGGLAHSAPLPAATAHPAAAAHAAHHPAVHHPILPPAAAAAAAAAAAAAVSSASLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGASGGFQHWGGMPCPCSMCQVPPPHHHVSAMGAGSLPRLTSDAK

• Full Name: oligodendrocyte lineage transcription factor 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC047511
• Gene Symbol: OLIG2
• Gene ID (NCBI): 10215
• RRID: AB_2881876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13516
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Publications

What published studies show

OLIG2 Monoclonal Antibody (1E8C5), catalog number 66513-1-Ig, has 24 citations published in Science, Cell Metabolism, Nucleic Acids Research, Theranostics, Advanced Science, Science Advances, Journal of Neuroinflammation, Free Radical Biology and Medicine, Communications Biology, International Immunopharmacology, Experimental Neurology, iScience, Brain Research Bulletin, Journal of Biological Chemistry, Experimental Cell Research, Frontiers in Oncology, Neuroscience, Phytotherapy Research, Biochemical Pharmacology, and Cell Biochemistry and Function. Research fields include neurodegeneration, glioma, demyelination, oligodendrocyte biology, stroke, and spinal cord injury.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Science; Targeting formyl peptide receptor 1 reduces brain inflammation and neurodegeneration; Authors - Yulin Li
		Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
human	WB	Cell Metab; Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation; Authors - Jian Zhou
human	WB	Cell Metab; Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2; Authors - Deguan Lv
human	WB	Nucleic Acids Res; A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma; Authors - Xianli Du
human	WB	Theranostics; Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2; Authors - Zirong Chen