OLIG2 Monoclonal antibody

OLIG2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

1E8C5

Cat no : 66513-1-Ig

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

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Validation Data Gallery

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  WB analysis using 66513-1-Ig

  Various tissues were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66513-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 66513-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 66513-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66513-1-Ig (OLIG2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66513-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66513-1-Ig (OLIG2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66513-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66513-1-Ig (OLIG2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66513-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66513-1-Ig (OLIG2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, human cerebellum tissue, rat cerebellum tissue, mouse brain tissue
• Positive IHC detected in: human brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:350-1:1400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Product Information

66513-1-Ig targets OLIG2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OLIG2 fusion protein Ag18838
• Full Name: oligodendrocyte lineage transcription factor 2
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 35-39 kDa
• GenBank accession number: BC047511
• Gene symbol: OLIG2
• Gene ID (NCBI): 10215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Publications

Species	Application	Title
mouse	IF	Glia; Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation; Authors - Wenjing Luo
mouse	IF	Front Cell Dev Biol; Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.; Authors - Kang Guo
human	WB	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
mouse	IHC,IF	Front Aging Neurosci; Effect of β-hydroxybutyrate on behavioral alterations, molecular and morphological changes in CNS of multiple sclerosis mouse model; Authors - Wei Sun
rat	IF	Exp Neurol; Role of TREM-1 in the development of early brain injury after subarachnoid hemorrhage.; Authors - Xin-Gang Sun
human	WB,IF	iScience; Hypoxia-induced HMGB1 promotes glioma stem cells self-renewal and tumorigenicity via RAGE; Authors - Cuifang Ye