|Positive WB detected in||human liver tissue|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11837-1-AP targets OLR1 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OLR1 fusion protein Ag2437|
|Full Name||oxidized low density lipoprotein (lectin-like) receptor 1|
|Calculated molecular weight||273 aa, 31 kDa|
|Observed molecular weight||48-52 kDa|
|GenBank accession number||BC022295|
|Gene ID (NCBI)||4973|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acid with the calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from glycosylation of four potential N-linked glycosylation sites located at C-terminal domain (PMID: 9052782).
|Product Specific Protocols|
|WB protocol for OLR1 antibody 11837-1-AP||Download protocol|
|IHC protocol for OLR1 antibody 11837-1-AP||Download protocol|
|IP protocol for OLR1 antibody 11837-1-AP||Download protocol|
|FC protocol for OLR1 antibody 11837-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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