ZNF830 Polyclonal antibody

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• ZNF830 Polyclonal antibody (25618-1-AP)

ZNF830 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25618-1-AP Copied!

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CCDC16, Coiled-coil domain-containing protein 16, OMCG1, SEL13, Zinc finger protein 830

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  WB analysis using 25618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25618-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 25618-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25618-1-AP (ZNF830 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 25618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25618-1-AP (ZNF830 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, rat testis tissue
• Positive IHC detected in: human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25618-1-AP targets ZNF830 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22538

  Product name: Recombinant human ZNF830 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 7-366 aa of BC011584

  Sequence: KRVINQEELRRLMKEKQRLSTSRKRIESPFAKYNRLGQLSCALCNTPVKSELLWQTHVLGKQHREKVAELKGAKEASQGSSASSAPQSVKRKAPDADDQDVKRAKATLVPQVQPSTSAWTTNFDKIGKEFIRATPSKPSGLTLLPDYEDEEEEEEEEEGDGERKRGDASKPLSDAQGKEHSVSSSREVTSSVLPNDFFSTNPPKAPIIPHSGSIEKAEIHEKVVERRENTAEALPEGFFDDPEVDARVRKVDAPKDQMDKEWDEFQKAMRQVNTISEAIVAEEDEEGRLDRQIGEIDEQIECYRRVEKLRNRQDEIKNKLKEILTIKELQKKEEENADSDDEGELQDLLSQDWRVKGALL

• Full Name: zinc finger protein 830
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011584
• Gene Symbol: ZNF830
• Gene ID (NCBI): 91603
• RRID: AB_2880163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NB3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

4.6 out of 5

3 verified customer reviews

What customers say

Customers report this antibody performs well for Western Blot, consistently detecting a major band at ~50 kDa across RKO, U2OS, and HEK293T lysates. Recommended dilutions range from 1:2000 to 1:5000, with incubation at 4°C overnight or 1 hour at room temperature. A few reviewers noted some weak, non-specific additional bands, but overall the antibody is described as reliable and effective, earning ratings of 4–5 stars.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Aleksandr (Verified Customer) (07-19-2025)

Antibody detect a major band at ~50 kDa with some weaker unspecific bands. Incubation was done overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

ZNF830 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:25618-1-AP)

Tsimafei (Verified Customer) (11-19-2024)

Overall antibody is great and detect a band ~ 50 kDa, though some weak additional bands are visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

ZNF830 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:25618-1-AP)

Moritz (Verified Customer) (08-16-2024)

Ab worked very well! WB, 1:5000, 1h@RT

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T lysate