ZNF830 Polyclonal antibody

ZNF830 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Reviews (2)

Cat no : 25618-1-AP

Synonyms

CCDC16, OMCG1, SEL13, zinc finger protein 830, ZNF830

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Validation Data Gallery

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  WB analysis using 25618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25618-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25618-1-AP (ZNF830 antibody at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25618-1-AP is ZNF830 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF830 fusion protein Ag22538
• Full Name: zinc finger protein 830
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011584
• Gene Symbol: ZNF830
• Gene ID (NCBI): 91603
• RRID: AB_2880163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (11-19-2024)

Overall antibody is great and detect a band ~ 50 kDa, though some weak additional bands are visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

ZNF830 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:25618-1-AP)

FH

Moritz (Verified Customer) (08-16-2024)

Ab worked very well! WB, 1:5000, 1h@RT

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T lysate